Each frame was lifted out and examined. They couldn't find the Queen but the eggs and drones were healthy which means the Queen is probably healthy too.
In this photo you can see the Queen cup, this is the beginning of a cell where the Queen may lay a fertile egg to raise another Queen.
At the moment there is only enough honey for the hive but later in the year they will be able to harvest some honey.
After throughly checking the hive it all seemed to be healthy and there are no parasites.
Thank you to Sonia for wearing a protective suit and taking the close up photos.